PubMed · 2956918
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P Y Castelain. 1987. [Dysidrosis].. https://pubmed.ncbi.nlm.nih.gov/2956918/
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Patients who are sensitive to fragrances should either use fragrance-free cosmetics or undergo a repeat open application test to the cosmetic or perfume to determine sensitivity. Unusual reactions include systemic contact dermatitis due to balsam of Peru, benzyl alcohol, and menthol. Some responses involve pigmented eruptions due to phototoxic or photoallergic agents in perfumes and incense. Other reactions include consort dermatitis and reactions to toothpastes, gum and perfumes in paper products, sanitary napkins, ostomy pastes, and detergents.
In this study we performed an in vitro assay of the effects of two gutta-percha formulations and one zinc oxide-eugenol and Canada balsam-based endodontic material on the behavior of a mixed cell population of human monocytes and lymphocytes. Cells were cultured either in direct contact with or near experimental samples of Ultrafil, standard gutta-percha cones or Endoseal and the inhibitory effects of these materials upon cell attachment and spreading on test surfaces were analyzed. Ultrafil and the standard gutta-percha cones showed little or no adverse effects, whereas the inhibitory effects of Endoseal appeared to be severe. There were statistically significant differences between the results obtained from both Ultrafil and gutta-percha cones and those obtained from Endoseal. Differences between all tested materials and their respective controls were also statistically significant.
Primary cultured cells derived from human pulp tissue were used for examining the cytotoxicity of nine commercial filling materials used in restoration and root canal procedures. Cell growth was evaluated by photo pattern analysis, cell nuclei counting, and neutral red uptake. L-strain cells were also used for comparison. Primary cultured cells from either the coronary or radicular part of the pulp tissue were less sensitive to the tested materials, than were L-cells. As for sensitivities over time following mixing of the materials, the cytotoxicity of the three restorative materials was immediately moderate but disappeared 24 hours after mixing. The other six root canal filling materials yielded similar moderate to high cytotoxic levels immediately after mixing and the effects continued. The order of cytotoxicity was: Diaket and Calvital (highest); Pulp canal sealer, AH-26, and Canals (high); and then Kloroperka and three restorative materials (moderate). The differences in cellular reactions between primary and strain cells was considered to be due to cell origin, i.e. diploid or heteroploid. The importance of using primary cultured cells from designated target tissues when evaluating various materials in vitro was discussed.