Molecular crowding on the cell surface.

T A Ryan; J Myers; D Holowka; B Baird; W W Webb

doi:10.1126/science.2962287

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PubMed · 2962287

Molecular crowding on the cell surface.

T A Ryan·J Myers·D Holowka·B Baird·W W Webb

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Abstract

Strong steric interactions among proteins on crowded living cell surfaces were revealed by measurements of the equilibrium spatial distributions of proteins in applied potential gradients. The fraction of accessible surface occupied by mobile surface proteins can be accurately represented by including steric exclusion in the statistical thermodynamic analysis of the data. The analyses revealed enhanced, concentration-dependent activity coefficients, implying unanticipated thermodynamic activity even at typical cell surface receptor concentrations.

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Animals Cell Membrane Membrane Fluidity Membrane Proteins Rats Receptors, Fc Receptors, IgE Thermodynamics Tumor Cells, Cultured Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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T A Ryan, J Myers, D Holowka, B Baird, W W Webb. 1988-01-01. Molecular crowding on the cell surface.. https://doi.org/10.1126/science.2962287

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