A mouse tRNA pseudogene derived from a tRNA(Trp) coding sequence.

R Ammendola; T Russo; F Cimino

PubMed · 2969875

A mouse tRNA pseudogene derived from a tRNA(Trp) coding sequence.

Abstract

The identification and characterization of a mouse tRNA(Trp) pseudogene is reported. A synthetic oligonucleotide (31 mer), identical with the 3' half of a tRNA(Trp), was used to examine three mouse lambda clones that are known to contain clusters of tRNA genes. A fragment of one of these lambda clones strongly hybridizes to the oligonucleotide; the sequence of this region shows the presence of a gene significantly homologous to the chick tRNA(Trp). However two point mutations and a three base deletion prevent the folding of a possible transcript of this gene. The presence of a conserved promoter sequence for RNA polymerase III, that should allow the transcription of this gene, does not ensure the transcription of the gene, at least in our in vitro system.

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Animals Bacteriophage lambda Cloning, Molecular Codon Electrophoresis, Polyacrylamide Gel HeLa Cells Humans Mice Molecular Sequence Data Oligonucleotides Phosphorus Radioisotopes Pseudogenes RNA, Transfer, Amino Acid-Specific RNA, Transfer, Trp Transcription, Genetic Journal Article Research Support, Non-U.S. Gov't

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R Ammendola, T Russo, F Cimino. A mouse tRNA pseudogene derived from a tRNA(Trp) coding sequence.. https://pubmed.ncbi.nlm.nih.gov/2969875/

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