An automated Western blot analysis using the phastsystem.

B Prieur; F Russo-Marie

doi:10.1016/0003-2697(88)90453-8

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An automated Western blot analysis using the phastsystem.

B Prieur·F Russo-Marie

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Abstract

We have developed a rapid, automated method for preparing Western blots of very small amounts of proteins, utilizing a commercially available electrophoresis system (Phastsystem, Pharmacia). This system has been adapted to transfer to nitrocellulose experimental samples that were initially separated in the same system by gradient-sodium dodecyl sulfate gel electrophoresis. The developing unit of the Phastsystem has permitted automation of all the necessary steps including incubation with antibodies, saturation of nonspecific binding sites, and washing. The total elapsed time for protein separation, transfer, and staining is about 6 to 7 h.

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Animals Annexins Autoanalysis Autoradiography Blotting, Western Electrophoresis, Polyacrylamide Gel Glycoproteins Immunoglobulins Rabbits Journal Article

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B Prieur, F Russo-Marie. 1988-08-01. An automated Western blot analysis using the phastsystem.. https://doi.org/10.1016/0003-2697(88)90453-8

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