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A simple purification method for a glucosyltransferase complex from Streptococcus mutans OMZ 176 with a high yield.

M Furutani·M Iwaki·T Yagi·M Iida·K Horiike·M Nozaki
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Abstract

1. Glucosyltransferase was purified from Streptococcus mutans OMZ 176 by ammonium sulfate fractionation and gel filtration. 2. The overall purification was about 15,000-fold with a yield of 53%. 3. The purified enzyme appeared to be a multienzyme complex consisting of three different components with molecular weights of 158,000, 153,000 and 149,000, respectively.

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Ammonium SulfateChromatographyChromatography, GelGlucosyltransferasesMolecular WeightPolysaccharides, BacterialStreptococcusJournal ArticleResearch Support, Non-U.S. Gov't

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M Furutani, M Iwaki, T Yagi, M Iida, K Horiike, M Nozaki. 1988. A simple purification method for a glucosyltransferase complex from Streptococcus mutans OMZ 176 with a high yield.. https://doi.org/10.1016/0020-711x(88)90238-8

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