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PubMed · 2982945

Activated human B cells display a functional IL 2 receptor.

R Mittler·P Rao·G Olini·E Westberg·W Newman·M Hoffmann·G Goldstein
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Abstract

These studies deal with the expression of a functional IL 2 receptor on activated primary human B cells. Antibody against the receptor (alpha-TAC) reacted with 25 to 65% activated B cells, inhibited B cell proliferation by 50% and inhibited B cell secretion of Ig by greater than 90%. These effects were shown to be independent of contaminating T lymphocytes. Anti-TAC immunoprecipitated a molecule of identical size (65,000 daltons) from T and B lymphocytes; B cells were also shown to actively synthesize the IL 2 receptor. The chymotryptic peptide chromatograms of TAC antigen from T and B cells show these molecules to be indistinguishable.

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Antibodies, MonoclonalAntigen-Antibody ReactionsAntigens, SurfaceB-LymphocytesBinding, CompetitiveChymotrypsinComplement System ProteinsHemolytic Plaque TechniqueHumansInterleukin-2Lymphocyte ActivationPeptide FragmentsPrecipitin TestsReceptors, ImmunologicReceptors, Interleukin-2Tumor Necrosis Factor Receptor Superfamily, Member 7Journal ArticleResearch Support, U.S. Gov't, P.H.S.

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R Mittler, P Rao, G Olini, E Westberg, W Newman, M Hoffmann, G Goldstein. 1985. Activated human B cells display a functional IL 2 receptor.. https://pubmed.ncbi.nlm.nih.gov/2982945/

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