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Sequences essential for IS50 transposition. The first base-pair.

C Sasakawa·S H Phadnis·G F Carle·D E Berg
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Abstract

Sequences near the ends of the insertion element IS50 are essential for its transposition, probably because they serve as sites upon which the IS50-encoded transposase protein acts. To determine if these essential sequences include the first base-pair at each end of IS50 we generated 5'C to 5'G transversions at these positions. Each mutation reduced the transposition frequency to 1% to 2% of wild-type. DNA sequence analyses showed that the mutant 5'G is preserved during transposition.

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Bacteriophage lambdaBase SequenceDNA Restriction EnzymesDNA Transposable ElementsDNA, ViralMutationPlasmidsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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C Sasakawa, S H Phadnis, G F Carle, D E Berg. 1985-04-20. Sequences essential for IS50 transposition. The first base-pair.. https://doi.org/10.1016/0022-2836(85)90235-9

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