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PubMed · 2991285

Multiple mRNAs for 3-hydroxy-3-methylglutaryl coenzyme A reductase determined by multiple transcription initiation sites and intron splicing sites in the 5'-untranslated region.

G A Reynolds·J L Goldstein·M S Brown
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Abstract

The current studies show that mRNAs with 16 different 5'-untranslated regions (varying in length from 68 to 670 nucleotides) are produced from the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene in hamster UT-1 cells. This complex pattern of mRNAs results from a combination of multiple transcription initiation sites and multiple 5' splice donor sites for the intron in the 5'-untranslated region of the gene. Analysis of the multiple mRNAs was made possible by a modification of the S1 nuclease technique in which we used a series of progressively truncated uniformly labeled, single-stranded [32P]DNA probes in addition to the usual end-labeled 32P-probes. All of the reductase mRNAs are diminished when UT-1 cells are incubated with sterols, indicating that all of them are subject to coordinate control.

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AnimalsBase SequenceCell LineCloning, MolecularCricetinaeDNADNA Restriction EnzymesEndonucleasesHydroxymethylglutaryl CoA ReductasesPlasmidsProtein BiosynthesisRNA SplicingRNA, Double-StrandedRNA, MessengerSingle-Strand Specific DNA and RNA EndonucleasesTranscription, GeneticJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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G A Reynolds, J L Goldstein, M S Brown. 1985-08-25. Multiple mRNAs for 3-hydroxy-3-methylglutaryl coenzyme A reductase determined by multiple transcription initiation sites and intron splicing sites in the 5'-untranslated region.. https://pubmed.ncbi.nlm.nih.gov/2991285/

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