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PubMed · 2993074

Paramagnetic probes of multicomponent electron-transfer systems.

H Kamin·C Batie·J D Lambeth·J Lancaster·L Graham·J C Salerno
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Abstract

Electron-transfer systems associated with steroid hormone production in the adrenals and with photosynthetic NADP+ reduction in the chloroplast share some important features. Complexes between components in both systems affect the exposure of prosthetic groups to water-soluble paramagnetic groups. The sensitivity of the components to probes of different charges suggests that electrostatic interactions are important in complex formation. Haem plane orientation in cytochrome P-450 of the adrenal system is parallel to the membrane plane in both native and reconstituted systems.

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Adrenal GlandsAnimalsCytochrome P-450 Enzyme SystemEdetic AcidElectron Spin Resonance SpectroscopyElectron TransportEndoplasmic ReticulumFerredoxin-NADP ReductaseFerredoxinsMitochondriaSteroid HydroxylasesJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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H Kamin, C Batie, J D Lambeth, J Lancaster, L Graham, J C Salerno. 1985. Paramagnetic probes of multicomponent electron-transfer systems.. https://doi.org/10.1042/bst0130615

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