Crystallization and preliminary diffraction data for iso-1-cytochrome c from yeast.

C Sherwood; G D Brayer

doi:10.1016/0022-2836(85)90192-5

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Crystallization and preliminary diffraction data for iso-1-cytochrome c from yeast.

C Sherwood·G D Brayer

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Abstract

Deep red crystals of the electron transfer protein, iso-1-cytochrome c from yeast (Saccharomyces cerevisiae), have been obtained from a 90% saturated solution of (NH4)2SO4 containing 2 mg protein/ml, 0.1 M-sodium phosphate and adjusted to pH 6.7. The space group is P4(1)2(1)2 (or P4(3)2(1)2) with a = b = 36.4 A, c = 136.8 A and Z = 8. Crystals are stable for at least ten days in the X-ray beam and diffract to better than 2.0 A resolution. Comparable and morphologically similar crystal forms of three iso-1-cytochrome c mutants at Phe87, a pivotal residue in the electron transport chain, have also been obtained.

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Crystallography Cytochrome c Group Cytochromes c Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Journal Article Research Support, Non-U.S. Gov't

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C Sherwood, G D Brayer. 1985-09-05. Crystallization and preliminary diffraction data for iso-1-cytochrome c from yeast.. https://doi.org/10.1016/0022-2836(85)90192-5

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