Polymyxin B use does not ensure endotoxin-free solution.

M J Kluger; R Singer; S M Eiger

doi:10.1016/0022-1759(85)90073-0

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Polymyxin B use does not ensure endotoxin-free solution.

M J Kluger·R Singer·S M Eiger

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Abstract

Polymyxin B is often added to in vitro samples to 'ensure' that endotoxin activity is removed. We present data, from the standard rabbit pyrogen test and the Limulus amebocyte lysate assay, that polymyxin B bound to a gel support will bind some, but not all, endotoxin. These data, in conjunction with previously published data by Morrison and Curry (1979), indicate that those studies that have relied on polymyxin B to inactivate endotoxin must be re-evaluated.

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Chromatography, Affinity Endotoxins Limulus Test Polymyxin B Polymyxins Journal Article Research Support, Non-U.S. Gov't

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M J Kluger, R Singer, S M Eiger. 1985-10-24. Polymyxin B use does not ensure endotoxin-free solution.. https://doi.org/10.1016/0022-1759(85)90073-0

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