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PubMed · 2999084

F'-coded, temperature-sensitive lambda cI857 repressor gene for easy construction and regulation of lambda promoter-dependent expression systems.

M Mieschendahl·B Müller-Hill
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Abstract

We describe the construction and properties of an F' factor which carries the temperature-sensitive cI857 allele of the repressor gene of coliphage lambda and which lacks the lambda cro function. This episome can easily be transferred to any F- and F' Escherichia coli strain, thus facilitating the construction and regulation of lambda promoter-dependent expression systems without the use of defective prophages.

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Bacteriophage lambdaDNA Restriction EnzymesElectrophoresis, Polyacrylamide GelGene Expression RegulationGenes, RegulatorGenes, ViralPlasmidsRepressor ProteinsTemperatureTranscription FactorsJournal Article

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M Mieschendahl, B Müller-Hill. 1985. F'-coded, temperature-sensitive lambda cI857 repressor gene for easy construction and regulation of lambda promoter-dependent expression systems.. https://doi.org/10.1128/jb.164.3.1366-1369.1985

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