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PubMed · 2999117

Hydroxyl radical attack on dopamine.

A Slivka·G Cohen
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Abstract

Hydroxyl radicals were generated in the presence of 1 mM dopamine (3,4-dihydroxyphenylethylamine) at pH 7.2 (50 mM phosphate buffer) by the following two mechanisms: 1) a classic Fenton-type reaction between hydrogen peroxide and a ferrous chelate (ferrous diethylenetriaminepentaacetate) and 2) the cyclical redox reactions of iron-EDTA/ascorbate. Three ring-monohydroxylated products of dopamine were detected by high performance liquid chromatography with electrochemical detection: 2-hydroxydopamine, 5-hydroxydopamine, and 6-hydroxydopamine in an approximate ratio of 3:2:1. Scavengers of hydroxyl radicals (dimethyl sulfoxide, mannitol, ethanol) suppressed the yields of products in a concentration-dependent manner. The formation of nonphysiologic hydroxylated forms of dopamine can provide a probe for the formation of hydroxyl radicals in dopamine neurons.

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Ascorbic AcidChemical PhenomenaChemistryChromatography, High Pressure LiquidDopamineEdetic AcidHydroxidesHydroxydopaminesHydroxyl RadicalOxidopamineJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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A Slivka, G Cohen. 1985-12-15. Hydroxyl radical attack on dopamine.. https://pubmed.ncbi.nlm.nih.gov/2999117/

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