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PubMed · 3000664

Modulation of E-receptor expression on activated T lymphocytes.

S K Oh·W L Farrar·F W Ruscetti
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Abstract

Our study indicated that the newly synthesized E-receptor, as measured with 125I-labeled monoclonal anti-E receptor antibody, on activated T lymphocytes were responsible for forming stable E-rosettes at 37 degrees C. Maximum induction of new E-receptor expression required at least 50 hr of culture with polyclonal T-cell activators, phytohemagglutinin, or phorbol myristate acetate. Polyclonal B-cell activator, lipopolysaccharide were not able to induce new E-receptor expression on the surface of T lymphocytes. The expression of the new E-receptor paralleled with the induction of Tac antigen expression. Interleukins 1 and 2 or Interferon-gamma were not able to initiate the induction of new E-receptors. However, a neuropeptide, endorphin exhibited biphasic effect on modulating the E-receptor expression, in the absence of polyclonal activators. As is the case with Tac antigen expression, induction of new E-receptor antigen may be a marker for activated T lymphocytes.

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AnimalsAntigens, SurfaceHumansKineticsLymphocyte ActivationLymphokinesPhytohemagglutininsProstaglandinsProtein BiosynthesisRabbitsRadioimmunoassayReceptors, ImmunologicRosette FormationT-LymphocytesTetradecanoylphorbol AcetateTumor Necrosis Factor Receptor Superfamily, Member 7Journal Article

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BibTeXRIS

S K Oh, W L Farrar, F W Ruscetti. 1986. Modulation of E-receptor expression on activated T lymphocytes.. https://doi.org/10.1016/0090-1229(86)90122-4

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