A silica-based reversed-phase column for single-stranded oligodeoxyribonucleotides.

K Makino; H Ozaki; H Wada; T Takeuchi; M Ikehara; T Fukui; H Sasaki; Y Kato

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PubMed · 3003710

A silica-based reversed-phase column for single-stranded oligodeoxyribonucleotides.

K Makino·H Ozaki·H Wada·T Takeuchi·M Ikehara·T Fukui·H Sasaki·Y Kato

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Abstract

A novel, silica-based, reversed-phase column targeted to the separation of single-stranded oligodeoxyribonucleotides has been developed by grafting monochlorooctadecylsilane onto silica which is a base material of reversed-phase columns (TSK 120A and 120T), in which polychlorosilane is used as a grafting reagent. By applying a shallow gradient elution of an aqueous acetonitrile solution containing 0.1 M ammonium acetate, chromatography with this column produced well-resolved peaks for large samples such as those having bases up to 26.

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Chromatography, High Pressure Liquid Indicators and Reagents Oligodeoxyribonucleotides Silicon Dioxide Journal Article

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K Makino, H Ozaki, H Wada, T Takeuchi, M Ikehara, T Fukui, H Sasaki, Y Kato. 1985. A silica-based reversed-phase column for single-stranded oligodeoxyribonucleotides.. https://pubmed.ncbi.nlm.nih.gov/3003710/

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