Endonucleolytic activity that cleaves immunoglobulin recombination sequences.

T J Hope; R J Aguilera; M E Minie; H Sakano

doi:10.1126/science.3003919

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PubMed · 3003919

Endonucleolytic activity that cleaves immunoglobulin recombination sequences.

T J Hope·R J Aguilera·M E Minie·H Sakano

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Abstract

An endonucleolytic activity has been identified in nuclear extracts of chick embryo bursa and mouse fetal liver cells. The activity introduces a double-strand cut in the vicinity of the recombination site of immunoglobulin joining gene segments. The cleavage occurs at the dinucleotide pair TG-AC. This activity is a good candidate for the putative endonuclease involved in recombination of the immunoglobulin variable, diversity, and joining regions. It is distinct from the endonuclease activities previously reported by others.

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Animals Base Sequence Bursa of Fabricius Chick Embryo Endonucleases Immunoglobulin J-Chains Immunoglobulin Variable Region Immunoglobulins Liver Mice Recombination, Genetic Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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T J Hope, R J Aguilera, M E Minie, H Sakano. 1986-03-07. Endonucleolytic activity that cleaves immunoglobulin recombination sequences.. https://doi.org/10.1126/science.3003919

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