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PubMed · 3004429

Calmodulin-like protein from Bacillus subtilis.

I J Fry·L Villa·G D Kuehn·J H Hageman
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Abstract

The first example of a calmodulin-like activity in a Gram-positive bacterium, Bacillus subtilis, is reported. A calcium ion-dependent, 3', 5' cyclic-AMP phosphodiesterase-stimulating activity was found in the soluble fraction of cell-free extracts of cells sporulating in a chemically-defined medium; activation was reversed by trifluoperazine. The activity was heat stable, bound to phenothiazine-agarose in a calcium ion-dependent manner and was eluted therefrom with buffer containing EGTA, and displaced authentic beef brain calmodulin from its antibody in a radioimmunoassay.

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3',5'-Cyclic-AMP PhosphodiesterasesAnimalsBacillus subtilisCalciumCalmodulinCattleMyocardiumRadioimmunoassayTrifluoperazineJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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I J Fry, L Villa, G D Kuehn, J H Hageman. 1986-01-14. Calmodulin-like protein from Bacillus subtilis.. https://doi.org/10.1016/0006-291x(86)90549-8

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