Nucleic acid chromatography: substitute solid support material for RPC-5 columns.

J M Flanagan; R K Fujimura; K B Jacobson

doi:10.1016/0003-2697(86)90096-5

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Nucleic acid chromatography: substitute solid support material for RPC-5 columns.

J M Flanagan·R K Fujimura·K B Jacobson

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Abstract

Chromatography of tRNA and DNA fragments on columns of reverse-phase 5 (RPC-5) exchange material has been widely employed for analytical and preparative studies. The Plaskon bead that formed the solid support on which a quaternary amine was absorbed is no longer commercially available. A Voltalef bead is available and provides similar, though not identical, chromatograms for Asp-tRNA, Ser-tRNA, and certain DNA fragments. Procedures are described for preparation of the column packing and for long-term operation of the column.

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Chromatography, High Pressure Liquid DNA DNA Restriction Enzymes DNA, Viral Deoxyribonucleases, Type II Site-Specific RNA, Transfer RNA, Transfer, Amino Acyl Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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J M Flanagan, R K Fujimura, K B Jacobson. 1986. Nucleic acid chromatography: substitute solid support material for RPC-5 columns.. https://doi.org/10.1016/0003-2697(86)90096-5

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