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Dose pipecolic acid interact with the central GABA-ergic system?

R Bernasconi·R S Jones·H Bittiger·H R Olpe·J Heid·P Martin·M Klein·P Loo·A Braunwalder·M Schmutz
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Abstract

Several previous studies have suggested a strong GABA-mimetic action of the endogenous brain imino acid, L-pipecolic acid (L-PA). In the present study, these observations were evaluated using electrophysiological and neurochemical methods. In contrast to published data our electrophysiological studies on rat cortical neurones in situ showed only a weak, but bicuculline-sensitive depressant action of L-PA on cortical neurones. Furthermore, L-PA proved to have no affinity for any of the three components of the GABA-benzodiazepine-chloride channel receptor complex. However, using a modification of published methods a weak affinity for the GABA-B receptor site was demonstrated (IC50 = 1.8 X 10(-3) M). L-PA showed no anticonvulsive activity in several tests; in particular, it did not protect mice from seizures induced by inhibition of L-glutamate-1-decarboxylase (EC 4.1.1.15: GAD). L-PA had a very weak action on brain GABA levels of mice, and did not modify the rate of GABA synthesis. In conclusion, these results are not compatible with a strong in vivo interaction between L-PA and GABA-mediated inhibitory transmission.

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AnimalsAnticonvulsantsBrainBrain ChemistryMaleMicePipecolic AcidsRatsReceptors, GABA-ASeizuresSynaptic Transmissiongamma-Aminobutyric AcidJournal Article

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R Bernasconi, R S Jones, H Bittiger, H R Olpe, J Heid, P Martin, M Klein, P Loo, A Braunwalder, M Schmutz. 1986. Dose pipecolic acid interact with the central GABA-ergic system?. https://doi.org/10.1007/bf01243346

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