Coliphage M13 cloning system and ddXTP chain-termination method for DNA sequencing.

Y P Qi

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PubMed · 3027889

Coliphage M13 cloning system and ddXTP chain-termination method for DNA sequencing.

Y P Qi

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Abstract

Two DNA fragments of cytochrome b gene in yeast mitochondrial DNA were sequenced by Messing's M13 cloning system and Sanger's ddXTP chain-termination method. M13mp8 and M13mp9 serve as vectors for insert fragment. The recipient strain is E. coli JM103 for transfection. When the ratio of insert/vector was 3:1, high frequencies of recombination and positive recombination were obtained. The two fragments, which have 575 bp and 709 bp, were sequenced. To read more bases, the ratio of ddXTP/dXTP must be reduced. On one X-ray film of 80 x 17 cm 394 bp were read.

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Base Sequence Coliphages DNA Transposable Elements DNA, Mitochondrial DNA, Recombinant Genetic Vectors Yeasts Journal Article

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Y P Qi. 1986. Coliphage M13 cloning system and ddXTP chain-termination method for DNA sequencing.. https://pubmed.ncbi.nlm.nih.gov/3027889/

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