Decrease of cellular ATP by dihexanoylglycerol may limit responses to protein kinase C activation.

J Dawson; N T Thompson; R W Bonser; H F Hodson; L G Garland

doi:10.1016/0014-5793(87)80036-4

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Decrease of cellular ATP by dihexanoylglycerol may limit responses to protein kinase C activation.

J Dawson·N T Thompson·R W Bonser·H F Hodson·L G Garland

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Abstract

1,2-sn-Dihexanoylglycerol (HHG) reduced the ATP content of HL-60 cells. This concentration-related (10-100 microM) effect reached a maximum of over 90%, was enantiomerically specific and not accompanied by release of lactate dehydrogenase. Oleoylacetylglycerols (3-100 microM) had no effect on ATP levels while phorbol dibutyrate (PDBu, 0.01-1 microM) decreased ATP content of HL-60 cells by up to 40%. Responses stimulated by HHG became limited as the concentration was increased above 10 microM, this being manifest as either a low maximum response compared to PDBu (superoxide release) or a bell-shaped concentration-effect curve (degranulation). HHG (30-100 microM) inhibited PDBu-stimulated superoxide release, this inhibition being enantiomerically specific. It is probable that the effect of HHG on ATP content impairs cellular responses mediated through protein kinase C activation.

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Adenosine Triphosphate Cell Line Diglycerides Enzyme Activation Glycerides Humans Phorbol 12,13-Dibutyrate Phorbol Esters Protein Kinase C Superoxides Journal Article

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J Dawson, N T Thompson, R W Bonser, H F Hodson, L G Garland. 1987-04-06. Decrease of cellular ATP by dihexanoylglycerol may limit responses to protein kinase C activation.. https://doi.org/10.1016/0014-5793(87)80036-4

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