PubMed · 3034723
[Insertion mutagenesis in Bacillus subtilis (Marmur)].
Abstract
A method for obtaining mutations in Bacillus subtilis using integration into the chromosome of the plasmid pHV60 ligated to chromosomal DNA fragments was developed. Auxotrophic mutants acquire, in addition, chloramphenicol-resistance, due to insertion of appropriate plasmid determinants. Chromosomal localization was established and the properties of several mutants were studied.
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M M Stoilova-Disheva, A A Prozorov. 1987. [Insertion mutagenesis in Bacillus subtilis (Marmur)].. https://pubmed.ncbi.nlm.nih.gov/3034723/
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