Heparin-binding angiogenesis factors: detection by immunological methods.

M G Wadzinski; J Folkman; J Sasse; K Devey; D Ingber; M Klagsbrun

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PubMed · 3040319

Heparin-binding angiogenesis factors: detection by immunological methods.

M G Wadzinski·J Folkman·J Sasse·K Devey·D Ingber·M Klagsbrun

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Abstract

Immunological methods for the detection of basic fibroblast growth (FGF) are described. Polyclonal antibodies directed against synthetic peptides representing amino-, internal, and carboxy-terminal regions of basic FGF, were raised in rabbits. Five techniques, enzyme-linked immunosorbent assay, immunoblot, electrophoretic transfer (Western) blot, immunoprecipitation and radioimmunoassay, were used to detect basic FGF. These techniques were used to demonstrate that a human hepatoma cell line synthesizes a growth factor structurally related to brain and pituitary basic FGF.

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Angiogenesis Inducing Agents Antibodies Carcinoma, Hepatocellular Cell Line Fibroblast Growth Factors Growth Substances Heparin Humans Immunoassay Liver Neoplasms Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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M G Wadzinski, J Folkman, J Sasse, K Devey, D Ingber, M Klagsbrun. 1987. Heparin-binding angiogenesis factors: detection by immunological methods.. https://pubmed.ncbi.nlm.nih.gov/3040319/

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