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A low-priced computer-assisted densitometer and analysis software for quantitative autoradiography.

J I Choca·F M Clark·R D Green·H K Proudfit
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Abstract

A computer-assisted densitometer which consists of a darkroom enlarger, a black-and-white exposure meter, an amplifier, an analog-to-digital converter, and a microcomputer with a monitor and a graphic printer can be utilized for the quantitative analysis of autoradiograms. QUANTAR, a computer program written in BASIC, records the density measurements and stores the data in a file that can be easily retrieved by commercially available spreadsheet software. A spreadsheet template was designed to convert the digital readings into concentration of ligand in tissue. A variety of spreadsheet templates can be created to analyze data for a standard curve, a Scatchard plot, or a binding competition curve. This system, which is easy to assemble, may be useful to the frugal investigator with a modest equipment budget.

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AdenosineAnimalsAutoradiographyDensitometryDiagnosis, Computer-AssistedReceptors, PurinergicSoftwareSubstantia GelatinosaJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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BibTeXRIS

J I Choca, F M Clark, R D Green, H K Proudfit. 1988. A low-priced computer-assisted densitometer and analysis software for quantitative autoradiography.. https://doi.org/10.1016/0165-0270(88)90058-1

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