PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix.

C L Bethea; T K Borg

doi:10.1016/0303-7207(88)90145-1

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PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix.

C L Bethea·T K Borg

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Abstract

PC12 cells on plastic grow as a single-layered lawn of cells which synthesize, store and secrete dopamine. In contrast, PC12 cells cultured on Englebreth-Holm-Swarm (EHS) tumor-derived extracellular matrix grow into multicellular aggregates. Matrix dissolution and cell migration appear to follow aggregate formation. PC12 cell plating efficiency is decreased on EHS-matrix but the doubling time of cells on EHS-matrix is comparable to plastic. Dopamine secretion and cellular content determined with a radioenzymatic assay as well as dopamine synthesis determined with cation-exchange chromatography are similar on a per cell basis in cultures of PC12 cells on plastic and EHS-matrix.

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Animals Cell Aggregation Cell Line Dopamine Extracellular Matrix Microscopy, Electron, Scanning Microscopy, Phase-Contrast Pheochromocytoma Sarcoma, Experimental Tumor Cells, Cultured Journal Article Research Support, U.S. Gov't, P.H.S.

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C L Bethea, T K Borg. 1988. PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix.. https://doi.org/10.1016/0303-7207(88)90145-1

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