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PubMed · 3072011

Rat sperm protamine. Isolation and sequence analysis.

H Ammer·A Henschen
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Abstract

Rat protamine was extracted from S-(pyridylethylated) epididymal sperm cell nuclei with dilute hydrochloric acid. The final purification was achieved by reversed-phase high-performance liquid chromatography. The primary structure was determined by N-terminal sequencing of the total S-(pyridylethylated) protein, and of endoproteinase Lys-C- and thermolysin-derived fragments. Rat protamine consists of 50 amino-acid residues. It is a typical type 1 protamine and differs in two and ten positions from the corresponding mouse and rabbit protamine, respectively. Only 26 positions are invariant in all type 1 mammalian protamines.

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Amino Acid SequenceAnimalsChromatography, High Pressure LiquidElectrophoresis, Polyacrylamide GelHumansMaleMolecular Sequence DataPeptide FragmentsPeptide HydrolasesProtaminesRatsRats, Inbred StrainsSpecies SpecificitySpermatozoaComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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H Ammer, A Henschen. 1988. Rat sperm protamine. Isolation and sequence analysis.. https://doi.org/10.1515/bchm3.1988.369.2.1301

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