A fluorogenic, mixed-disulfide reagent for thiol assay.

K J Willis; F W Teale

doi:10.1016/0003-2697(86)90101-6

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PubMed · 3085537

A fluorogenic, mixed-disulfide reagent for thiol assay.

K J Willis·F W Teale

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Abstract

The design and synthesis of the mixed disulfide (ortho-nitroaniline-N-ethyldithio) 2-[pyridyl-5-thioureido-N'-(5-fluorescein)] are described and the chemical and spectroscopic properties of this thiol-specific fluorogenic reagent are presented. The high reactivity and sensitivity of this reagent in thiol assay are demonstrated with low-molecular-weight thiols and with human carbonmonoxyhemoglobin and its subunits. Comparison with conventional absorption methods shows that at least 100 times less material is needed; moreover, high background absorbance or turbidity do not interfere with the assay.

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Carboxyhemoglobin Energy Transfer Fluoresceins Humans Kinetics Molecular Weight Spectrometry, Fluorescence Sulfhydryl Compounds Sulfhydryl Reagents Thiourea Journal Article

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K J Willis, F W Teale. 1986. A fluorogenic, mixed-disulfide reagent for thiol assay.. https://doi.org/10.1016/0003-2697(86)90101-6

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