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Colony formation of cytolytic T cells in semisolid medium.

D Collavo·H Engers·M Nabholz
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Abstract

Addition of supernatant from concanavalin A-stimulated spleen cells to in vitro primed cytolytic T lymphocytes in semisolid medium stimulated the growth of colonies of cytolytic lymphocytes. Optimal results were obtained using a peritoneal adherent cell underlayer where a 10% plating efficiency (greater than or equal to 4 cells per colony) was achieved when between 5000 and 100 000 cells were plated per dish. Individual colonies were harvested and tested in a short term (5h) 51Cr release microassay, employing 200 target cells. The frequency of lytic colonies varied from 46--67%. The observed lytic activities were specific for the relevant allogeneic target cells.

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AnimalsCells, CulturedClone CellsCrosses, GeneticCulture MediaCytotoxicity Tests, ImmunologicCytotoxicity, ImmunologicFemaleMiceMice, Inbred AKRMice, Inbred C57BLMice, Inbred DBASpleenT-LymphocytesJournal Article

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D Collavo, H Engers, M Nabholz. 1978. Colony formation of cytolytic T cells in semisolid medium.. https://doi.org/10.1002/eji.1830080811

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