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An enzyme immunoassay for osteocalcin.

H Tanaka·M Kuwada·M Shiraki·K Katayama
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Abstract

A sensitive and specific enzyme immunoassay for bovine osteocalcin was developed with the use of enzyme-labeled antigen. Bovine osteocalcin was conjugated with beta-D-galactosidase for use in the immunoassay. The minimum amount of osteocalcin detectable by this method was 5-10 pg/assay. The enzyme immunoassay could detect immunoreactive osteocalcin levels in normal human sera, giving a value of 6.58 +/- 0.72 ng/ml (mean +/- SE of 16 human subjects). The correlation coefficient between the levels measured by this method and radioimmunoassay was 0.94.

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AnimalsCalcium-Binding ProteinsCattleHumansImmunoenzyme TechniquesMaleOsteocalcinRabbitsRadioimmunoassaybeta-GalactosidaseJournal Article

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H Tanaka, M Kuwada, M Shiraki, K Katayama. 1986-11-20. An enzyme immunoassay for osteocalcin.. https://doi.org/10.1016/0022-1759(86)90210-3

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