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PubMed · 3104459

Nucleotide sequence of a cDNA clone encoding a rabbit immunoglobulin-lambda light chain: the V lambda region differs markedly from that of other species.

D J Hayzer·J C Jaton
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Abstract

A cDNA clone (pDH7) has been isolated which encodes the entire leader peptide and variable (V) region and most of the constant (C) region of a rabbit lambda-light chain. Although similar to amino acid sequences derived from fragments of isolated lambda-chains from several Basilea rabbits, differences in the first framework region (FR1) suggest that at least two germ-line V lambda genes are expressed. There are major differences between rabbit V lambda sequences and light chains of other species: in particular, rabbit lambda-chains have an additional four amino acids in the vicinity of the FR2-CDR2 junction. The same region also has significant homology with the human D2 germ-line mini-gene sequence, especially with a 14-nucleotide sequence previously shown to be homologous to human and rabbit heavy chain CDR2 sequences. Similar homologies in other heavy and light chain sequences suggest that D-gene segments may be derived from VH genes, perhaps by transposition. The framework regions of the rabbit lambda-chain encoded by clone pDH7 show the greatest homologies with those of human kappa- and lambda-sequences (46 to 54% homology), with that of chicken sequence (55%), and least with murine V lambda sequences (40%).

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Amino Acid SequenceAnimalsAntigens, BacterialBase SequenceCloning, MolecularDNAImmunoglobulin Constant RegionsImmunoglobulin Variable RegionImmunoglobulin lambda-ChainsImmunoglobulinsPolysaccharides, BacterialRNA, MessengerRabbitsComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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D J Hayzer, J C Jaton. 1987-04-01. Nucleotide sequence of a cDNA clone encoding a rabbit immunoglobulin-lambda light chain: the V lambda region differs markedly from that of other species.. https://pubmed.ncbi.nlm.nih.gov/3104459/

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