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Expression of immunoglobulin lambda chains in the laboratory rat.

D Zhou·G A Leslie·K Guo·G A Gutman
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Abstract

We immunized a BALB/c mouse with the lambda-bearing rat IgG1 myeloma IR31, fused its spleen cells with the hybridoma parent line P3.X63.Ag8.653, and isolated a monoclonal antibody (G33/11) directed against rat immunoglobulin lambda chains. We used this antibody to classify two existing rat hybridomas as lambda-bearing proteins (D4.37HL.252 and PC61.5), and isolated one new lambda-bearing rat IgM hybridoma, G36/1. All the normal inbred rat sera that were tested contained lambda-bearing Ig as detected by G33/11, at levels ranging from 1.5% to 13% of the total serum Ig, the mean value being 7.9%. This antibody will be valuable for broadening our understanding of the immunogenetics of the rat, and for the characterization of monoclonal antibodies made in this species.

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AnimalsAntibodies, MonoclonalEnzyme-Linked Immunosorbent AssayImmunoglobulin GImmunoglobulin kappa-ChainsImmunoglobulin lambda-ChainsMiceMice, Inbred BALB CRatsRats, Inbred StrainsSpecies SpecificityJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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D Zhou, G A Leslie, K Guo, G A Gutman. 1986. Expression of immunoglobulin lambda chains in the laboratory rat.. https://doi.org/10.1111/j.1744-313x.1986.tb01114.x

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