A new variable region in mouse immunoglobulin lambda light chains.

P Sanchez; P A Cazenave

doi:10.1084/jem.166.1.265

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A new variable region in mouse immunoglobulin lambda light chains.

P Sanchez·P A Cazenave

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Abstract

A series of lambda+ murine hybridomas were derived from a BALB/c mouse after a single injection of anti-lambda 2 antibodies coupled to LPS. Nine lambda B cell clones (five lambda 2 and four lambda 3) were expected and seven reacted with antibodies specific for the C lambda 2 constant region but showed a particular isoelectric spectrum. Their RNA products did not hybridize with the V lambda probe. The partial DNA sequence of gene segments coding the unexpected light chain of one hybridoma shows that the V gene segment has only 55% homology with the V lambda 2 gene segment sequence and that J lambda 2 and probably C lambda 2 gene segments are used. Taken together, these results demonstrate the existence of a new lambda light chain.

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Animals Antibodies, Anti-Idiotypic Base Sequence DNA Hybridomas Immunoglobulin Variable Region Immunoglobulin lambda-Chains Isoelectric Focusing Mice Mice, Inbred BALB C Nucleic Acid Hybridization RNA, Messenger Sequence Homology, Nucleic Acid Journal Article

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P Sanchez, P A Cazenave. 1987-07-01. A new variable region in mouse immunoglobulin lambda light chains.. https://doi.org/10.1084/jem.166.1.265

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