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Should platelets be radiolabelled in plasma medium?

M F Tsan·R L Hill-Zobel
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Abstract

Because of the low labelling efficiency of platelets with In-111 oxine in plasma containing media, most investigators have labelled platelets in plasma-free media. However, labelling of platelets with In-111 oxine in plasma-free media may result in either: irreversible damage leading to rapid clearance from the circulation and permanent hepatic sequestration; or reversible damage with transient hepatic sequestration without affecting the in vivo survival. Since human platelets can be labelled with In-111 oxine in plasma in sufficient efficiency for routine clinical use, they should be labelled in plasma whenever possible. The use of newer In-111 chelates, tropolone and mercaptopyridine-N-oxide, which label platelets with high efficiency in plasma as well as in plasma-free media, may offer another alternative.

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AnimalsBlood PlateletsCell SurvivalHumansIndium RadioisotopesIsotope LabelingOrganometallic CompoundsOxyquinolinePlasmaPyridinesRabbitsThionesTropoloneJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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M F Tsan, R L Hill-Zobel. 1987. Should platelets be radiolabelled in plasma medium?. https://doi.org/10.1002/ajh.2830250318

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