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PubMed · 3117433

Liquid chromatography and fluorescence polarization immunoassay methods compared for measuring flecainide acetate in serum.

R J Straka·T J Hoon·R L Lalonde·J A Pieper·M B Bottorff
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Abstract

We analyzed 99 patients' serum samples for concentrations of a new antiarrhythmic agent, flecainide acetate, by fluorescence polarization immunoassay (FPIA) and "high-performance" liquid chromatography (HPLC). Within-day and between-day coefficients of variation at concentrations in the low and high ends of the therapeutic range were less than 7% for HPLC and less than 9% for FPIA. There was no statistical difference in the mean (+/- SD) concentrations of the clinical serum samples measured by the two methods (607 +/- 334 micrograms/L by HPLC, 602 +/- 344 micrograms/L by FPIA), but results by each differed by a mean of 0.13%. FPIA and HPLC measurements correlated significantly (r = 0.98, P less than 0.05), and were linearly related (slope = 0.970, intercept = 13 micrograms/L) as assessed by orthogonal regression. Both assay methods produced similar concentration measurements and were sufficiently accurate and precise to be used in therapeutic drug monitoring.

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Anti-Arrhythmia AgentsChromatography, High Pressure LiquidChromatography, LiquidFlecainideFluorescence PolarizationHumansImmunologic TechniquesComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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R J Straka, T J Hoon, R L Lalonde, J A Pieper, M B Bottorff. 1987. Liquid chromatography and fluorescence polarization immunoassay methods compared for measuring flecainide acetate in serum.. https://pubmed.ncbi.nlm.nih.gov/3117433/

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