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Chicken apolipoprotein A-I: cDNA sequence, tissue expression and evolution.

L Byrnes·C C Luo·W H Li·C Y Yang·L Chan
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Abstract

Using an antibody against chicken apolipoprotein (apo) A-I, we identified multiple cDNA clones for the protein in two intestinal cDNA libraries in lambda gt11. The complete nucleotide sequence of chicken apoA-I cDNA was determined. The sequence predicts a mature protein of 240 amino acids, a 6-amino acid propeptide and an 18-amino acid signal peptide. Using a 32P-cDNA probe, we detected the presence of apoA-I mRNA in 21 day old chicken intestine, liver, kidney, spleen, breast muscle and brain. The primary sequence of apoA-I contains numerous tandem repeats of 11 and 22 residues in a manner similar to the mammalian proteins. Our analysis of apoA-I sequences from human, rabbit, dog, rat, and chicken indicates that the rate of amino acid substitution is considerably faster in the rat lineage than in other mammalian lineages.

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Amino Acid SequenceAnimalsApolipoprotein A-IApolipoproteins ABase SequenceBiological EvolutionChickensCloning, MolecularDNAGenesHumansIntestinal MucosaMolecular Sequence DataOrgan SpecificitySequence Homology, Nucleic AcidSpecies SpecificityTranscription, GeneticComparative StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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L Byrnes, C C Luo, W H Li, C Y Yang, L Chan. 1987-10-14. Chicken apolipoprotein A-I: cDNA sequence, tissue expression and evolution.. https://doi.org/10.1016/0006-291x(87)91137-5

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