A useful method for determining class-specific rheumatoid factors with computerized analyses of data.

P L Gigase; C H Bridts; L S De Clerck; W J Stevens

doi:10.1016/0022-1759(88)90014-2

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A useful method for determining class-specific rheumatoid factors with computerized analyses of data.

P L Gigase·C H Bridts·L S De Clerck·W J Stevens

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Abstract

A simple and sensitive enzyme-linked immunosorbent assay (ELISA) was developed to determine rheumatoid factors (RFs) of IgG, IgA and IgM class. Standardisation was performed with a standard reference serum for IgM-RF, calibrated according to the WHO preparation, and with the serum of a patient containing high levels of IgA- and IgG-RF. The sigmoidal shaped calibration curve was fitted with a computerized four parameter logistic model with simplified mathematical computations. This method provided to be more accurate for measuring RF levels, as judged by the smaller residuals, than logit or log-linear transformations. The considerable reduction in processing time, which is obtained by the computerized analysis of data, makes this method of class-specific RF determination suitable for routine analysis.

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Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin A Immunoglobulin G Immunoglobulin M Pepsin A Rheumatoid Factor Software Journal Article

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P L Gigase, C H Bridts, L S De Clerck, W J Stevens. 1988-02-24. A useful method for determining class-specific rheumatoid factors with computerized analyses of data.. https://doi.org/10.1016/0022-1759(88)90014-2

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