PubMed · 3126953
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J G Collee. 1988-03-12. Testing time for side room tests.. https://doi.org/10.1136/bmj.296.6624.733
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A simple method that allows easy identification of rifamycin B-producing strains is described. This method involves the use of an enzyme, rifamycin oxidase, which converts inactive rifamycin B to active rifamycin S. In this method, colonies to be tested are grown in pairs. The two colonies are then transferred to two plates seeded with a sensitive strain of Staphylococcus aureus, one plate of which contains the enzyme rifamycin oxidase. All paired colonies which show a larger inhibition zone diameter on the enzyme-containing plate are identified as rifamycin B producers.
The penicillin-phenol red acidometric test was found to be highly efficient and suitable for the detection of beta-lactamase production in staphylococci. The author presents his modification of the procedure and discusses its advantages. He recommends that all staphylococcal strains be tested for beta-lactamase production on the day of isolation.
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