Radioimmune double PEG precipitation technique for detecting complexed IgE.

K Meretey; U Böhm; A Falus; S Bozsoky

doi:10.1016/0022-1759(79)90247-3

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Radioimmune double PEG precipitation technique for detecting complexed IgE.

K Meretey·U Böhm·A Falus·S Bozsoky

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Abstract

The presence of IgE in immune complex-enriched fractions prepared by 3% polyethylene glycol (PEG) precipitation was studied. The dissolved PEG-insoluble fraction was incubated with [125I]anti-IgE antibodies an then precipitated again with 3% PEG. Radioactivity in the washed pellets was taken as indicating IgE present in immune complex form. A calibration curve was constructed using artificially prepared IgE-anti-IgE complexes. The method developed may be useful to detect other components present in immune complex fractions.

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K Meretey, U Böhm, A Falus, S Bozsoky. 1979. Radioimmune double PEG precipitation technique for detecting complexed IgE.. https://doi.org/10.1016/0022-1759(79)90247-3

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