Simple graphical methods for use with complex ligand-binding and enzyme mechanisms.

M J Crabbe

doi:10.1016/0014-5793(88)81259-6

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Simple graphical methods for use with complex ligand-binding and enzyme mechanisms.

M J Crabbe

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Abstract

Simple graphical plots in distribution-free space are shown to provide estimates of mechanism and kinetic parameters for complex steady-state processes. The methods were tested using simulated 1:1, 1:2, 2:2, 2:3 and 3:3 functions, and real data from polyol dehydrogenase, NADPH oxidation in the presence of DL-glyceraldehyde, and NADPH oxidation in the presence of an NADPH-binding protein. Estimates of mechanism and parameters agreed closely with those found from non-linear regression by computer.

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Aldehyde Reductase Animals Buffers Cattle Glyceraldehyde Kinetics Lens, Crystalline Mathematics NADP Oxidation-Reduction Phosphates Potassium Potassium Compounds Sugar Alcohol Dehydrogenases Journal Article Research Support, Non-U.S. Gov't

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M J Crabbe. 1988-08-01. Simple graphical methods for use with complex ligand-binding and enzyme mechanisms.. https://doi.org/10.1016/0014-5793(88)81259-6

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