The human Lyt-3 molecule requires CD8 for cell surface expression.

J P DiSanto; R W Knowles; N Flomenberg

doi:10.1002/j.1460-2075.1988.tb03221.x

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The human Lyt-3 molecule requires CD8 for cell surface expression.

J P DiSanto·R W Knowles·N Flomenberg

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Abstract

We have previously identified a monoclonal antibody, T8/2T8-5H7, which clustered serologically with CD8 monoclonal antibodies, but lacked reactivity with L cell transfectants expressing the human CD8 molecule (Lyt-2 homologue). Based on these observations, we postulated that T8/2T8-5H7 might recognize the human Lyt-3 gene product. To test this hypothesis, we have isolated a full-length cDNA encoding the human Lyt-3 molecule and have characterized its product in additional transfection experiments. The results of these studies indicate that the human Lyt-3 cDNA encodes a product recognized by the antibody T8/2T8-5H7. Interestingly, the human Lyt-3 molecule cannot be expressed alone, but requires the human Lyt-2 homologue for efficient cell surface expression. A heterodimer composed of the human Lyt-2 and Lyt-3 molecules may have importance in T cell-target cell interactions.

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Animals Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Antigens, Ly Antigens, Surface Base Sequence CD8 Antigens Cell Line Cell Membrane DNA Fluorescent Antibody Technique Gene Expression Regulation Humans L Cells Mice Molecular Sequence Data Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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J P DiSanto, R W Knowles, N Flomenberg. 1988. The human Lyt-3 molecule requires CD8 for cell surface expression.. https://doi.org/10.1002/j.1460-2075.1988.tb03221.x

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