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Immunochemical studies on pneumococcal type 37 capsular polysaccharide.

P Z Allen·W H Bowen
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Abstract

Pneumococcal type 37 capsular polysaccharide was obtained free of contaminants by affinity chromatography on Con-A, wheat germ agglutinin, Maclura pomifera lectin and HOPC-8 mouse myeloma protein affinity columns. The immunochemical reactivity of native and periodate oxidized borohydride reduced type 37 polysaccharide antigen with polyclonal rabbit and monoclonal mouse anti-Pn37 hybridoma antibodies was studied by quantitative precipitation. Quantitative hapten inhibition studies, employing the isomeric series of alpha- and beta-(1----2), (1----3), (1----4) and (1----6)-linked glucobioses as competitive inhibitors of antibody precipitation establish a specificity for anti-Pn37 antibody directed at least in part, against the Glc beta(1----2) Glc (sophorosyl) unit. A high mol. wt, D-glucose containing polysaccharide antigen, cross-reactive with rabbit anti-Pn37 is reported which was found to occur in the culture medium of 7 of 19 strains of Actinomyces examined.

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AnimalsBinding, CompetitiveChemical PhenomenaChemistryChromatography, AffinityCross ReactionsHaptensImmunodiffusionPolysaccharides, BacterialJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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P Z Allen, W H Bowen. 1988. Immunochemical studies on pneumococcal type 37 capsular polysaccharide.. https://doi.org/10.1016/0161-5890(88)90008-9

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