Crossmatching on platelets by flow cytometry.

G X Wang; G Y Terashita; P I Terasaki

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PubMed · 3154488

Crossmatching on platelets by flow cytometry.

G X Wang·G Y Terashita·P I Terasaki

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Abstract

1. HLA antigens on platelets can be detected by alloantisera used for typing lymphocytes. 2. There is a general correlation between reactivity of sera against platelets and reactivity against T cells. However, some SLE patients have broadly reactive antibodies to T cells that are much less reactive to platelets. These sera may contain IgG autoantibody to non-HLA T-cell autoantigens. 3. The Pl-FCXM may be both more sensitive than the conventional cytotoxic crossmatching techniques and more discriminating than the T-FCXM in detecting relevant deleterious antibodies. All 11 patients with a negative platelet FXCM had functioning kidneys at 1 month, whereas 7 of 12 patients with a positive Pl-FCXM had nonfunctional kidneys at 1 month (p = 0.003). 4. It is suggested that the Pl-FCXM offers a simple, sensitive, and effective method of crossmatching without the high false positive rate of T-FCXM.

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Blood Platelets Evaluation Studies as Topic False Positive Reactions Flow Cytometry Graft Rejection HLA Antigens HLA-A2 Antigen Histocompatibility Testing Humans Isoantibodies Kidney Function Tests Kidney Transplantation Outcome and Process Assessment, Health Care T-Lymphocytes Comparative Study Journal Article

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G X Wang, G Y Terashita, P I Terasaki. 1988. Crossmatching on platelets by flow cytometry.. https://pubmed.ncbi.nlm.nih.gov/3154488/

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