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PubMed · 3160459

Competitive protein-binding assay for trimetrexate.

J C Drake·C J Allegra·G A Curt·B A Chabner
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Abstract

A competitive protein-binding assay has been developed for trimetrexate (TMTX) based on the tight binding of this drug to human dihydrofolate reductase (tetrahydrofolate dehydrogenase). In this assay, TMTX competes with 3H-methotrexate for binding to the enzyme. Free drug is separated from that bound to reductase by adsorption with dextran-albumin-coated charcoal. TMTX is measurable over a range from 2 X 10(-9) to 5 X 10(-8) M in plasma, with a coefficient of variation of less than 10%. Measurements of TMTX in plasma, cerebrospinal fluid, and urine agreed closely with parallel determinations in aqueous solutions.

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AnimalsMaleMiceQuinazolinesRadioligand AssayTetrahydrofolate DehydrogenaseTrimetrexateJournal Article

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J C Drake, C J Allegra, G A Curt, B A Chabner. 1985. Competitive protein-binding assay for trimetrexate.. https://pubmed.ncbi.nlm.nih.gov/3160459/

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