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PubMed · 3169160

Method for growing primitive erythroid progenitors (BFU-E) from rat bone marrow.

N Basara·L Biljanović-Paunović·V Pavlović-Kentera
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Abstract

A method for growing erythroid bursts from rat bone marrow cells is presented. The methylcellulose culture technique with rat spleen-conditioned medium was used. Successful growth of rat BFU-E was obtained with 20% fetal bovine serum when anemic rat plasma was used as the source of erythropoietin. Plating efficiency was 60 BFU-E per 2 x 10(5) nucleated cells seeded. The proliferative activity determined by cytosine arabinoside (Ara-C) suicide was about 22%. The effect of chronic hypertransfusion on erythroid colony formation is presented.

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AnimalsBone Marrow CellsCell AggregationColony-Forming Units AssayCulture MediaErythroblastsErythrocyte CountErythropoiesisFemaleHematopoietic Stem CellsRatsRats, Inbred StrainsJournal ArticleResearch Support, Non-U.S. Gov't

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N Basara, L Biljanović-Paunović, V Pavlović-Kentera. 1988. Method for growing primitive erythroid progenitors (BFU-E) from rat bone marrow.. https://pubmed.ncbi.nlm.nih.gov/3169160/

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