PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 3170897

Streptococcal adherence on various restorative materials.

J Satou·A Fukunaga·N Satou·H Shintani·K Okuda
View original source ↗Export metadata ↓

Abstract

The adherence of Streptococcus sanguis ATCC 10556, S. sanguis ATCC 10557, S. mutans Ingbritt, and S. mutans OMZ 176 to the surfaces of composite resins, amalgam alloys, and a Au-Ag-Pd alloy was measured. Adhesion was correlated with values for hydrophobicity and zeta-potential of the bacteria and the restorative materials. The hydrophobicity of the restoratives showed a positive correlation between the numbers of adherent S. sanguis cells, suggesting that hydrophobic interactions are important for the adherence of this bacterial species. In contrast, the numbers of adherent S. mutans cells showed a positive correlation with the zeta-potential of the restoratives, suggesting that electrostatic interactions are important in adherence of this bacterium.

Explore related subjects

Bacterial AdhesionChemical PhenomenaChemistry, PhysicalComposite ResinsDental AlloysDental AmalgamStreptococcus mutansStreptococcus sanguisSurface PropertiesJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

J Satou, A Fukunaga, N Satou, H Shintani, K Okuda. 1988. Streptococcal adherence on various restorative materials.. https://doi.org/10.1177/00220345880670031301

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2026

Parallel evolutionary trajectories rewire enteropathogenic Escherichia coli adhesion to restore host attachment.

Enteropathogenic Escherichia coli (EPEC) causes disease in children, presenting as chronic diarrhea that can impair physical and cognitive development. The attachment of typical EPEC (tEPEC) to the gut epithelium via bundle-forming pili (BFP) is a key factor in its virulence. Yet, infections by atypical EPEC (aEPEC), which lack BFP, have become increasingly common. To investigate how aEPEC recover host-attachment in the absence of BFP, we performed experimental evolution using a non-adherent E. coli, constructed to mimic the ancestor of aEPEC, and selected adherent progeny. Highly adherent variants evolved through phase-variable activation of type I fimbriae (T1F), followed by two alternative trajectories: bacterial filamentation, which increases T1F avidity, or point mutations in the T1F adhesin FimH that enhance ligand affinity. Extending our analysis to the genomes of 327 aEPEC strains isolated from infected patients revealed that similar FimH mutations are common. We further demonstrated experimentally that these naturally occurring variants often increase epithelial-attachment. Our findings implicate T1F in aEPEC pathogenesis and suggest it may be clinically relevant for anti-adhesion therapy. More broadly, these results indicate that impaired host-attachment can be rapidly compensated by upregulating and optimizing an alternative adhesin, and that combining experimental evolution with comparative genomics can reveal evolutionary trajectories occurring in nature.

Bacterial Adhesion↗
PubMed2026

Trimeric autotransporter adhesins driving chain-like adhesion diversify surface colonization strategies in Shiga toxin-producing Escherichia coli.

Bacteria frequently colonize host and environmental surfaces under fluid flow. Chain-like adherence pattern (CLAP) is an EibG-mediated surface colonization phenotype of certain Shiga toxin-producing Escherichia coli (STEC) that lack the locus of enterocyte effacement (LEE). EibG, an immunoglobulin-binding trimeric autotransporter adhesin, drives CLAP, but the temporal dynamics and genetic diversity underlying chain formation remain unclear. Here, we use live-cell time-lapse imaging to show that chains arise from single cells that elongate and divide without separation. Under flow, chains resist detachment and undergo shear-dependent fragmentation at cell-cell junctions, releasing viable clonal units that disperse downstream. Comparative genomics reveals diversity among EibG-related adhesins and identifies distinct lineages, including chain-like adhesins (Cla) that mediate CLAP while lacking IgG binding. Screening of 1,354 genomes from England shows that claB is present in 95.6% of strains from major LEE-negative STEC serotypes, highlighting its epidemiological prevalence. Targeted mutagenesis demonstrates that chain formation and IgG binding are mediated by distinct structural domains, revealing the modular functional architecture of these adhesins. Furthermore, we show that EibG, ClaA, and ClaB confer robust resistance to complement-mediated killing. Collectively, these findings establish CLAP as a dynamic, surface-associated strategy of LEE-negative STEC and reveal diversification among adhesins that drive this behavior.

Bacterial Adhesion↗
PubMed2007

Biofilm formation by Staphylococcus epidermidis on nitrogen ion implanted CoCrMo alloy material.

Staphylococcus epidermidis is the primary cause of medical device-related infections due to its adhesion and biofilm forming abilities on biomaterial surfaces. For this reason development of new materials and surfaces to prevent bacterial adhesion is inevitable. In this study, the adhesion of biofilm forming S. epidermidis strain YT-169a on nitrogen (N) ion implanted as well as on as-polished CoCrMo alloy materials were investigated. A medical grade CoCrMo alloy was ion implanted with 60 keV N ions to a high dose of 1.9 x 10(18) ions/cm(2) at substrate temperatures of 200 and 400 degrees C. The near-surface implanted layer crystal structures, implanted layer thicknesses, and roughnesses were characterized by XRD, SEM and AFM. The number of adherent bacteria on the surfaces of N implanted specimens was found to be 191 x 10(6) CFU/cm(2) for the 200 degrees C and 70 x 10(6) CFU/cm(2) for the 400 degrees C specimens compared to the as-polished specimen (3 x 10(6) CFU/cm(2)). The adhesion test results showed that S. epidermidis strain YT-169a adhere much more efficiently to the N implanted surfaces than to the as-polished CoCrMo alloy surface. This was attributed mainly to the rougher surfaces associated with the N implanted specimens in comparison with the relatively smooth surface of the as-polished specimen.

Bacterial Adhesion↗