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Modifications of a method for preparing retinal wholemounts for sectioning.

R Colello
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Abstract

A method for embedding and sectioning retinal wholemounts is described. It employs a hydrophilic embedding agent, LR White, and coverslips inert to standard embedding resins. This simple technique, which has worked successfully on both enzymatically and autoradiographically labelled material, provides a means by which retinal wholemounts can be cut nearly parallel to the plane of the retinal layers. Previously prepared retinal wholemounts can also be successfully embedded and sectioned more than a year later by the method described.

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Acrylic ResinsAnimalsIn Vitro TechniquesMicrotomyRatsRetinaJournal ArticleResearch Support, Non-U.S. Gov't

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R Colello. 1988. Modifications of a method for preparing retinal wholemounts for sectioning.. https://doi.org/10.3109/10520298809107181

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