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Protection against complement-mediated cell damage by Ca2+ and Zn2+.

K J Micklem·G M Alder·C D Buckley·J Murphy·C A Pasternak
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Abstract

Ca2+ and Zn2+ prevent antibody-dependent complement-induced permeability changes in tonsil lymphocytes and Lettre cells. Lactate dehydrogenase leaks out from Lettre cells at high complement:cell ratios, under which conditions higher concentrations of Ca2+ and Zn2+ are required for protection. Ca2+ and Zn2+ do not inhibit complement activation or C9 binding to Lettre cells, and prevent leakage through preformed lesions. It is concluded that the extent of complement-induced membrane damage depends on the concentration of extracellular Ca2+, and may be modulated by changes in extracellular Ca2+ or Zn2+.

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AnimalsCalciumCell Membrane PermeabilityCell SurvivalCells, CulturedComplement C3Complement C9Complement Inactivator ProteinsComplement System ProteinsHemolysisLymphocytesMiceZincJournal ArticleResearch Support, Non-U.S. Gov't

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K J Micklem, G M Alder, C D Buckley, J Murphy, C A Pasternak. 1988. Protection against complement-mediated cell damage by Ca2+ and Zn2+.. https://doi.org/10.1159/000463048

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