Induction of multiple cytochrome P-450 species in housefly microsomes--SDS-gel electrophoresis studies.

R H Stanton; F W Plapp; R A White; M Agosin

doi:10.1016/0305-0491(78)90179-7

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Induction of multiple cytochrome P-450 species in housefly microsomes--SDS-gel electrophoresis studies.

R H Stanton·F W Plapp·R A White·M Agosin

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Abstract

1. Microsomal fractions isolated from various housefly strains have been characterized with respect to multiple forms of cytochrome P-450 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. Susceptible NAIDM houseflies were pretreated with known inducers of cytochrome P-450, and their microsomal electrophoretic profiles were compared to control NAIDM microsomes, using as standards partially purified cytochrome P-450s from noninduced NAIDM houseflies. 3. Tentatively, at least five different species of cytochrome P-450 may exist in the NAIDM housefly strain. 4. A comparison of the microsomal electrophoretic profile of different housefly strains also indicates the presence of at least two additional cytochrome P-450 species. 5. Induction with alpha-pinene and phenobarbital was expressed by a shift of the maximum absorbance at 452 nm in the CO-difference spectrum to lower wavelengths in the NAIDM strain; whereas, beta-naphthoflavone, although increasing the amount of cytochrome P-450, did not change the wavelength of maximum absorbance. 6. Cytochromes of the P-452 type appear to predominate in the susceptible NAIDM strain, while cytochromes of the P-450 and P-448 types predominate in resistant strains.

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Animals Cytochrome P-450 Enzyme System Electrophoresis, Polyacrylamide Gel Houseflies Microsomes Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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R H Stanton, F W Plapp, R A White, M Agosin. 1978. Induction of multiple cytochrome P-450 species in housefly microsomes--SDS-gel electrophoresis studies.. https://doi.org/10.1016/0305-0491(78)90179-7

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