Plasmid detection and sizing in single colony lysates.

W M Barnes

doi:10.1126/science.318764

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PubMed · 318764

Plasmid detection and sizing in single colony lysates.

W M Barnes

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Abstract

A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.

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Colicins DNA, Bacterial DNA, Circular Electrophoresis, Agar Gel Escherichia coli Extrachromosomal Inheritance Methods Nucleic Acid Conformation Plasmids Journal Article

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W M Barnes. 1977-01-28. Plasmid detection and sizing in single colony lysates.. https://doi.org/10.1126/science.318764

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