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PubMed · 319610

A method for concentrating cerebrospinal fluid cells.

H W Kölmel
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Abstract

The morphology of CSF cells changes in vitro rapidly. An optimal cytologic evaluation therefore requires special demands for the cell preparation. The cell concentration by means of the forced spontaneous sedimentation yields the best cytomorphologic results. A simple method for cell concentration has been described. Its application in two variations is determined by the cell content. In the first version the cells sediment spontaneously on the microscopic slide and in the second version on the membrane filter. The sedimentation is facilitated by the absorbent paper. This method is particularly suited for concentrating cultured cells and CSF cells, which have been incubated in the presence of tracer.

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Cerebrospinal FluidChildCytological TechniquesHumansJournal Article

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H W Kölmel. A method for concentrating cerebrospinal fluid cells.. https://pubmed.ncbi.nlm.nih.gov/319610/

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Cerebrospinal fluid dissecting into spinal epidural space after lumbar puncture causing cauda equina syndrome: review of literature and illustrative case.

OBJECTS: We report a case of epidural cerebrospinal fluid (CSF) leak after lumbar puncture caused by CSF dissecting into the spinal epidural space. The incidence of this phenomenon may be higher than suspected, although most cases may remain asymptomatic. MATERIALS AND METHODS: A 4-year-old girl with new-onset seizure underwent a diagnostic lumbar puncture, the results of which were normal; 3 h later, she began experiencing severe low-pressure headaches and lower back pain, bilateral lower extremity weakness, numbness, and pain, and urinary retention when upright. Spinal MRI demonstrated extensive epidural CSF collection posterior to the thecal sac extending from the cervicothoracic junction to the sacrum. After 48 h in the supine position and gradual mobilization, the patient had complete resolution of symptoms and no neurological sequelae. CONCLUSION: Patients usually recover without any neurological deficits after conservative treatment. Prone or lateral decubitus positioning immediately after lumbar puncture may decrease the incidence of this phenomenon.

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